NaDES-based biorefinery of Spirulina (Arthrospira platensis): A new path for sustainable high value-added metabolites

To meet the criteria of modern extraction, it is essential to use solvents that simultaneously reach standards green chemistry and allow extraction a wide spectrum bio-compounds. Natural Deep Eutectic Solvents (NaDES), as solution, this difficult task represent satisfactory alternative organic solvents. Microalgae are recognized sustainable resource produce polar non-polar metabolites, such carotenoids, free fatty acids (FFA) phycobiliproteins. Among them, Spirulina (Arthrospira platensis) one most studied, due its strong potential for food, health cosmetic. In work, two biorefinery scenarios were investigated using NaDES. The first combination polar/non-polar sequential solid/liquid together with liquid–liquid extract. second scenario implements an innovative triphasic solid/liquid/liquid approach, based on simultaneous NaDES in step. Several screened study identify best pair. According results, approach allowed increase productivity by factor 4 chlorophylls FFA, 2 carotenoids. It was also interesting note phycocyanin-enriched fraction achieves much higher levels purity than corresponding single-phase both applied scenarios. These results pave way time microalgae entirely industrially credible.